Crystal structure of S. aureus Cas9 in complex with crRNA and its target DNA

In past posts, you asked tell in more detail about CRISPR / Cas9 and approaches to genome editing. I just did not include such a voluminous material in the last post. And in this I will not include, for example, the TALEN method, which can potentially be more accurate and has its advantages. But darling, yes.

Let's try to walk through the already well-described method of genetic modification using CRISPR-Cas9 and look a little more broadly at the prospects that it opens up for us. I was wildly interested in the direction of xenogeneic transplantation from pigs to humans. The first, "pre-correction" methods showed that such a pig transplant is killed by the body within a few minutes ... But the method was not discarded at all. Still, this is a promising way not to wait until the next potential organ donor dies, but to grow them in advance. Then it turned out that pigs also carry a pack of specific retroviruses that are built into their genome and can cause an epidemic of a new xenozoonosis. And viruses, too, must somehow be picked out before transplantation. And somewhere here people in white coats appear on the stage, doing something incomprehensible in their laboratories ...
KlauS 11 march 2021, 9:56