Crystal structure of S. aureus Cas9 in complex with crRNA and its target DNA
In past posts, you asked tell in more detail about CRISPR / Cas9 and approaches to genome editing. I just did not include such a voluminous material in the last post. And in this I will not include, for example, the TALEN method, which can potentially be more accurate and has its advantages. But darling, yes.
Let's try to walk through the already well-described method of genetic modification using CRISPR-Cas9 and look a little more broadly at the prospects that it opens up for us. I was wildly interested in the direction of xenogeneic transplantation from pigs to humans. The first, "pre-correction" methods showed that such a pig transplant is killed by the body within a few minutes ... But the method was not discarded at all. Still, this is a promising way not to wait until the next potential organ donor dies, but to grow them in advance. Then it turned out that pigs also carry a pack of specific retroviruses that are built into their genome and can cause an epidemic of a new xenozoonosis. And viruses, too, must somehow be picked out before transplantation. And somewhere here people in white coats appear on the stage, doing something incomprehensible in their laboratories ...
Not so long ago, my baby son asked me - what experiment do I consider the most important one conducted in the 20th century? He needed it for a school essay.
This simple question put me into a stupor for several hours. Because the choice was really difficult. With the nineteenth century it is easier - there it is immediately clear that the double-slit experiment *.
And with the twentieth?
Of course, if you choose by significance, then everything is simple. This "Trinity" - the world's first nuclear weapons test, which took place on July 16, 1945. Without exaggerating at all, we can say that after this test the world woke up different.
But, for all the significance of this experiment, it did not bring new knowledge. Before that, everyone knew that the bomb would explode. The experiment did not add any new knowledge.
On reflection, I decided that I should choose one of the social experiments - in the twentieth century, scientists first turned their attention to themselves - starting to study human behavior. For a person, understanding his nature is much more significant than understanding the structure of the atom.
Having considered the Stanford Prison Experiment, Milgram's experiment and Universe-25, aka Rat Paradise, I chose an experiment to breed the friendly fox of the Soviet scientist, Academician Dmitry Belyaev.
Who remembers the game called WipeOut, those will please Japanese movie from Japan Advanced Institute of Science and Technology(JAIST). They have made a model of WipeOut track in reality
Levitation is based on the Meissner Effect. Bolides are cooled with help of liquid nitrogen.
Сonsiderable interest arousing the bolides controlling system, but unfortunately we don't have any additional information